OVERVIEW

MSIsensor2 is an improved version of MSIsensor, featuring a large upgrade in the microsatellite instability (MSI) detection for tumor only or Cell-Free Tumor DNA (ctDNA) sequencing data. The original MSIsensor is specially designed for tumor/normal paired sequencing data.

RESULT

Given tumor only sequencing data, MSIsensor2 uses machine learning models to figure out the MSI status for a distribution per microsatellite. Finally, the msi score (number of msi sites / all valid sites) can be calculated. In our test of 117 EGA samples, results from tumor only module are comparable with paired tumor and normal sequencing data input (see two result figures below) . The recommended msi score cutoff value is 20% (msi high: msi score >= 20%). We also tested TCGA and EGA data whose results showed the accuracy of tumor only module is up to 99% and illustrated the comparable performance advantage of tumor only module over original MSIsensor tumor/normal paired module (see ROC figures below) . In addition, for the tumor only module, we also tested ctDNA sequencing data from different companies. The results showed that MSIsensor2 can accurately discriminate the microsatellite status of ctDNA sequencing samples.

Tumor Only vs Paired

CORRELATION COEFFICIENT

Correlation coefficient between the two modules is 0.94, that levels of the msi score of different samples perform similarly under the two modules.

High R-square

High R-square shows that even though the two modules use different algorithms, their results are highly consistent, which fully demonstrates that the result of our tumor only module is reliable.

MSIsensor2 vs TSO500

To further illustrate the accuracy of tumor only module in MSIsensor2, we deliberately customized a model for the TSO500-panel and tested it with 10 samples. The results are shown in the table on the right-hand side. It is not difficult to see that the model we customized for the TSO500 is consistent with its own ability to discriminate the microsatellite status of the sample. In addition, MSIsensor2 is not critical to sequencing methods or sequencing types, which refers that MSIsensor2 can handle both target gene sequencing data and amplification sequencing data, furthermore, it is applicable for WES, WGS, panel and ctDNA data.

FASTER!

By testing 117 samples from EGA with different types of sequencing data, in average, the tumor only module is 10 times faster than the paired module of MSIsensor. A typical WES data can be finished within 180 seconds.